|| 要旨トップ | 目次 |||日本生態学会第63回全国大会 (2016年3月、仙台) 講演要旨
一般講演（口頭発表） F2-14 (Oral presentation)
Over the last decade, the population of Yakushika (Cervus nippon yakushimae) has rapidly increased in Yakushima. Consequently, most understory species has decreased their abundance and biomass except for some non-palatable one. It is expected that Yakushika is now depending on non-palatable species. The purpose of this study was to test this possibility by examining stomach contents.
We sampled stomach contents of 90 individuals culled by hunters in 5 areas under different deer density. We extracted DNA from those samples and determined sequences of the rbcL region after PCR amplification using the next-generation sequencer (MiSEQ). Then we identified plant species by searching identical sequences with BLAST.
We identified 99 plant species (average 23 ± 6.34 species per individual) were detected from 90 individuals. Of which 25 and 21 species were palatable and non-palatable plants, respectively. This result showed that Yakushika depends on both palatable and non-palatable species under high density. Among 5 areas, the proportion of non-palatable species was the highest in the highest density area.