| 要旨トップ | 目次 | 日本生態学会第67回全国大会 (2020年3月、名古屋) 講演要旨
ESJ67 Abstract


一般講演(口頭発表) N02-09  (Oral presentation)

Study of aquatic food web based on high-precision stable carbon isotopic measurement of individual amino acids

*Yuchen SUN(Univ. Tokyo, JAMSTEC), Naoto F. ISHIKAWA(JAMSTEC), Hodaka KAWAHATA(Univ. Tokyo), Nanako O. OGAWA(JAMSTEC), Yoshinori TAKANO(JAMSTEC, Univ. Tokyo), Naohiko OHKOUCHI(JAMSTEC)

Tracing carbon transfer through food webs is a major challenge in ecological science. Recently, stable carbon isotopic composition (δ13C) of amino acids (AAs) has been increasingly used for tracing the source and flow of carbon in the ecosystem. It becomes a powerful tool for studies of food web structure, animal migration and habitat use. On the other hand, the application is still limited due to the experimental difficulties. We have developed an improved analytical method based on a multi-dimensional high-performance liquid chromatography (HPLC) and a nano-scale elemental analyzer/isotope-ratio mass spectrometry (EA/IRMS) for the high-precision δ13C measurement of proteinogenic AAs.
    Based on the new method, we analyzed a couple of representative species collected from northwest Pacific (spear squid, Heterololigo bleekeri and blue mackerel, Scomber australasicus). The δ13C of AAs in these two species showed a similar pattern. δ13C values varied over 30‰ with Phe and Leu being most 13C depleted and Gly being most enriched. The results support the hypothesis that some non-essential AAs like Gly in marine higher consumers are largely biosynthesized de novo.


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