The population of Rhizanthes lowii, Rafflesiaceae was first discovered in Gunung Pueh National Park, Sematan, Sarawak during the Scientific Expedition in 2015. R. lowii is a holoparasitic plant and relies on the host plant species in Tetrastigma, Vitaceae, for their resources. R. lowii is distributed in shaded areas, surrounded by boulders and near to small stream in Gunung Pueh National Park. Field observation and monthly monitoring were conducted during March 2016 – January 2017 to identify reproductive characteristics of R. lowii. The results of monitoring of a total of 19 flower buds and five blooming flowers over 11 months indicate that the flower buds of R. lowii had high mortality and low recruitment rate. Although sex of the flowers was not adequately determined, the species is likely unisexual. Initial diameter and height had significant effects on the growth rate of flower buds in R. lowii. Temperature also had significant effect on the recruitments. Preliminary findings from this research are important in understanding the reproductive characteristics, which will contribute to the better management plan and conservation of R. lowii.