| 要旨トップ | 目次 | 日本生態学会第68回全国大会 (2021年3月、岡山) 講演要旨
ESJ68 Abstract


一般講演(ポスター発表) P1-009  (Poster presentation)

環境DNAに基づく純淡水魚フクドジョウの系統地理推定 【B】
Phylogeographic analysis on Siberian stone loach based on environmental DNA 【B】

*八柳哲(北海道大学), 神戸崇(北海道大学), 水本寛基(北海道大学), 中嶋信美(国立環境研), 福島路生(国立環境研), 荒木仁志(北海道大学)
*Tetsu YATSUYANAGI(Hokkaido Univ.), Takashi KANBE(Hokkaido Univ.), Hiroki MIZUMOTO(Hokkaido Univ.), Nobuyoshi NAKAJIMA(NIES), Michio FUKUSHIMA(NIES), Hitoshi ARAKI(Hokkaido Univ.)

Since primary freshwater fishes depend exclusively on each freshwater system throughout the life histories, their phylogeographic structures often reflect historical processes of diversification and distribution. However, traditional phylogeographic approaches require a great deal of effort to collect many DNA samples from large geographical areas. In this study, we conducted a large-scale environmental DNA (eDNA) survey in Hokkaido to understand the geographic patterns of both species distributions and intra-genetic variations of freshwater fishes. Firstly, the species distributions were comprehensively evaluated by MiFish eDNA metabarcoding. Based on the metabarcoding result, we focused on the most-commonly detected fish, Siberian stone loach (Barbatula toni) and applied a newly-developed eDNA detection system to its intra-genetic variation and phylogeographic breaks. As a result, several lineages of B. toni were identified, which presumably reflects historical migratory patterns of this species in Hokkaido. This study illustrates effectiveness of the eDNA technique to characterize not only species distribution trends but also intra-genetic variations and phylogeographic patterns. With further analytical optimizations, this approach has the potential to advance phylogeographic studies for understanding historical diversification processes of the primary freshwater fishes and other various organisms without catching individuals.


日本生態学会