| 要旨トップ | 目次 | 日本生態学会第59回全国大会 (2012年3月,大津) 講演要旨
ESJ59/EAFES5 Abstract


一般講演(ポスター発表) P1-075A (Poster presentation)

cellular-level sex allocation in homothallic diatom

*Shirokawa, Y. (Univ. Tokyo), Shimada, M. (Univ. Tokyo), Wakamoto, Y. (Univ. Tokyo)

We studied sex allocation of isogenic unicellular homothallic centric diatom with the single-cell resolution under tightly controlled induction conditions. We designed a microfluidic system that allows to track cell divisions and sexual differentiation of multiple lineages in parallel by trapping cells in an array of micrometre-size chambers. Our study revealed that sex allocation of individual mother cells is affected by three factors: cell lineage, cell size and cell density. First, the sibling cell pairs showed the bias toward the same choice of sexual differentiation fates. Second, we found a significant negative correlation between the size of mother cell area and a gametangium sex ratio of 2 sibling cells. Third, the male-biased sex ratio declined as cell density within the microchamber increases. Together, these results have characterized multiple factors affecting the sex allocation of a homothallic unicelluar organism.


日本生態学会